The pluripotency involved factors, Lin28 and Notch1 are targets of neuronal miRNAs in hepatic stellate cells
J Huang, I Macheleidt, M Perrech, A Noetel, HP Dienes, J Hescheler, R Büttner, T Saric, M Odenthal · Zeitschrift für Gastroenterologie · 2015 · Heft 01
Bibliografische Angaben
Zusammenfassung
Background and Aim: Myofibroblastic hepatic stellate cells (HSC) are the central cell types of liver fibrosis due to their excessive matrix production. By deep sequencing of the Ago2 interacting transcriptome we have demonstrated that neuronal miR-9, miR-125b, miR -128 are involved in regulation of chemokine signaling during myofibroblastic HSC activation (Noetel et al., 2013). In the present study, we aimed to analyse further putative targets of the Ago2 neuronal miRNA complex, namely Notch1, KLF4, and Lin28. Methods: Notch1 and Lin28 gene expression was quantified in primary rat HSC cultures by real time PCR.…
