The effect of extended transportation time on the quality of freshly isolated human liver cells

Introduction: Given that the liver carries out a wide range of functions, such as detoxification, metabolism and homeostasis, isolated hepatocytes are important in vitro models for research questions to do with pharmacokinetics, toxicology etc. In particular, the use of human hepatocytes is important for the validation of studies done with animal models and for translational research. However, generally speaking, it is difficult for an individual researcher to isolate hepatocytes himself for experimentation due to the large amount of manpower required. This manpower is required to collect the donated remnant liv…