Genome wide DNA promoter methylation: Differences in human subcutaneous vs. omental visceral adipose tissue
M Keller, L Hopp, XL Liu, K Rohde, M Klös, A Dietrich, MR Schön, D Gärtner, T Lohmann, M Dreßler, M Stumvoll, P Kovacs, H Binder, M Blüher, Y Böttcher · Diabetologie und Stoffwechsel · 2015 · Heft 04
Bibliografische Angaben
Zusammenfassung
Background: Differences in DNA methylation pattern between subcutaneous adipose tissue (SAT) and omental visceral adipose tissue (OVAT) may help, together with corresponding changes in the mRNA profile, to elucidate variances in metabolic activity. In the present study we examined genome wide promoter methylation and mRNA expression pattern in 80 paired human SAT and OVAT samples including lean as well as obese subjects. Methods: DNA methylation analysis was performed using methylated DNA immunoprecipitation and subsequent hybridising on Affymetrix Human Promoter 1.0R tilling arrays. Expression profiles were gen…
