The role of TBC1D1 and TBC1D4 in contraction-induced glucose uptake in mouse skeletal muscle

Background and aims: The RabGTPase-activating proteins (RabGAPs) TBC1D1 and TBC1D4 (AS160) are key players in AKT- and AMPK-regulated glucose metabolism. The aim of this study is to analyze the contribution of these two homologous proteins to the contraction-mediated glucose uptake and metabolism in skeletal muscle. Methods: TBC1D1/TBC1D4-double-deficient mice (D1/4KO) were generated. Ex vivo muscle contraction was conducted using isolated Extensor digitorum longus (EDL) and Soleus muscles and contraction force and time for half-capacity were measured in a myograph chamber. Subsequently, contraction- and insulin…