Bile acid cocktail (BAC) induces similar changes in the expression of ion transporter MRNA in cultured esophageal epithelial cells (EEC) as observed in biopsies of Barrett's esoph…
D Laczkó, V Venglovecz, P Hegyi, Z Rakonczay, O Inczefi, R Róka, T Wittmann, A Rosztóczy · Zeitschrift für Gastroenterologie · 2015 · Heft 05
Bibliografische Angaben
Zusammenfassung
Introduction: Bile acids are thought to have an important role in the mucosal injury of the esophagus and though acid extruding mechanisms can be key factors of the defense. We aimed to determine the effect of a BAC on the activity of ion transporters and the expression of their mRNA in cultured EECs and compare these results with the observations obtained in biopsy samples of patients with BE. Patients, methods: In confluent monolayers of CP-A EECs intracellular pH (pHi) was measured with a fluorescent dye and Na+/H+ exchangers (NHEs), Na+/HCO3- cotransporter (NBC), Cl-/HCO3- exchanger (AE) activities were dete…
