Genome-wide chromatin profiling of Legionella pneumophila-infected human macrophages reveals activation of the pro-bacterial host factor TNFAIP2
I DuBois, A Marsico, W Bertrams, A Sittka-Stark, B Caffrey, M Schweiger, M Eberhardt, J Vera, M Vingron, B Schmeck · Pneumologie · 2016 · Heft 07
Bibliografische Angaben
Zusammenfassung
Introduction: Legionella pneumophila (L. pneumophila) is a causative agent of severe pneumonia. Infection leads to a broad host cell response, as evident e.g. on the transcriptional level. Chromatin modifications, which control gene expression, play a central role in the transcriptional response to L. pneumophila. Methods: We infected human blood-derived macrophages with L. pneumophila and used chromatin immunoprecipitation followed by sequencing (ChIP-Seq) to screen for gene promoters with the activating histone 4 acetylation mark (pan-acH4). We transferred these findings to investigate the effect of L. pneumop…
