Induction and secretion of pro-oncogenic heat shock protein 27 in ovarian cancer cells
M Weiss, MB Stope, G Klinkmann, D Könsgen, S Brucker, D Wallwiener, M Burchardt, A Mustea · Geburtshilfe und Frauenheilkunde · 2016 · Heft 10
Bibliografische Angaben
Zusammenfassung
Objective: This study examines the induction and secretion of pro-oncogenic heat shock protein 27 (HSP27) in ovarian cancer. Materials and methods: Protein expression was determined by Western blot analysis and enzyme-linked immunosorbent assay (ELISA). Results: The expression of HSP27 was evaluated by Western blot analysis in OVCAR-3 and SK-OV-3 cells. Interestingly, ELISA analysis of supernatant showed obvious amounts of secreted HSP27 in both cell lines. We further analysed the effects of HSP27 wt overexpression in OVCAR-3 cells on intra- and extracellular HSP27 amounts. Transient transfection with HSP27 wt w…
