Isolation of circulating tumor cells enables additional marker staining following panel sequencing
MHD Neumann, Y Decker, H Schneck, S Schömer, F Meier-Stiegen, T Fehm, D Niederacher, H Neubauer · Geburtshilfe und Frauenheilkunde · 2016 · Heft 10
Bibliografische Angaben
Zusammenfassung
Circulating tumor cells (CTC) are rare cells dissociated successfully from the primary tumor into the blood stream. The presence of CTCs is associated with increased risk to develop metastases and a short survival. Detection and isolation of CTCs is necessary. Here we show a workflow to isolate single CTCs for molecular characterization by combining the CellSearch and CellCelector. Single cells were classified as CTCs if nucleus (DAPI) and cytokeratin (FITC and/or TRITC) positive while CD45 (Cy5) negative. Detection and quantification of single cells from control samples and patient samples were performed using…
