Glycoproteomic Profiling of Hodgkin Lymphoma Reveals Novel Proteins that Contribute to the Microenvironment and Have Diagnostic Utility

Background: Proteins with N-linked glycosylation are frequently expressed on cell membranes and/or secreted, and represent idea biomarkers with diagnostic and therapeutic utility. In this study, we carried out unbiased identification of N-linked glycoproteins of Hodgkin lymphoma-derived cell lines. Design/Methods: Glycoproteomic analysis was carried out for 3 classical Hodgkin lymphoma (CHL) cell lines using solid-phase extraction of N-linked glycopeptides (SPEG) followed by liquid chromatography/tandem mass spectrometry. The expression of a subset of proteins was validated using western blot and immunohistochem…