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Stability of DNA methylation signatures in a long-term murine xenograft system of juvenile myelomonocytic leukemia

CF Krombholz, A Meier, S Fluhr, CM Niemeyer, M Erlacher, C Flotho · Klinische Pädiatrie · 2015 · Heft 07

Dokument
1200313
CareLit-ID
Jahr
2015
Publikation
PDF
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Metadaten
DOI
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Bibliografische Angaben

Zeitschrift
Klinische Pädiatrie
Autor:innen
CF Krombholz, A Meier, S Fluhr, CM Niemeyer, M Erlacher, C Flotho
Ausgabe
Heft 07 / 2015
Seiten
–
Erschienen: 2015-07-27 00:00:00
ISSN
0300-8630
DOI
10.1055/s-0035-1550252

Zusammenfassung

Introduction: Juvenile myelomonocytic leukemia (JMML) is a myeloproliferative disorder of early childhood that is resistant to conventional cancer therapies. Aggressive cases are characterized by specific DNA hypermethylation. Methods: Rag2-/- γc-/- mice were xenotransplanted with primary mononuclear cells from JMML patients. Methylation arrays (Illumina 450K) were used to compare DNA methylation patterns in source material and human leukocytes retrieved from xenografted mice. Results: Leukemic engraftment in mice reflected the clinical phenotype of JMML in many aspects (splenomegaly, cachexia, myeloid prolifera…

Schlagworte

Methylation Cells Patterns Juvenile Myelomonocytic Leukemia Epigenetic Leukemia-Initiating Stability Signatures Long-Term Murine Klinische Pädiatrie