Pivotal Role of the Serum- and Glucocorticoid-inducible kinase 1 in Rhabdomyosarcoma

Introduction: The serum-and-glucocorticoid-inducible-kinase-1 (SGK1) is ubiquitously expressed. Expression of SGK1 is normally low in most cells, but is dramatically upregulated under pathophysiological conditions, like hypertension, diabetes and tumor growth. SGK1 increases the store operated Ca2+ entry (SOCE), which is accomplished by the pore forming ion channel unit Orai1 and its regulator STIM1. Methods: Transcript levels of Orai1, STIM1 and SGK1 were determined by RT-PCR and Western blotting, as well as SOCE by Fura-2 fluorescence. Results: The transcript levels and protein abundance of Orai1, STIM1 and SG…