Imaged-guided Therapy of Ewing Sarcoma using targeting Nanoparticles encapsulated with Iron oxide and siRNA against EWS/FLI1

Ewing Sarcoma (ES) is the second most common bone tumour in children and adolescents. It is characterized by a specific translocation between the EWS gene and members of the ETS-family of transcription factors. The most important molecular target is the translocation between EWS and FLI1 [t(11;22)(q24;q12)] because it is identified in about 86% of all ES fusion transcripts. The objective of the project is to realise a save and efficient delivery of siRNA against EWS/FLI1 specifically into ES cells. Therefore we use Lipid-Protamin-Hyaluronic acid-Nanoparticles (LPH-NP) for complex formation of anionic siRNA and c…