Comparison of Four Different Methods for the Recovery of Dotriacontane-16,17-14C from Small Animal Respiratory Tissue

Summary The efficiency and accuracy of four different methods of tissue preparation for the recovery of dotriacontane-16,17-14C (DOT-16,17-14C) from rat respiratory tissue were compared by means of liquid scintillation counting. A DOT-16, 17-14C standard solution was placed on the respiratory epithelium of the nasal apex, larynx, trachea and main bronchi. Combustion of the lyophilized organs in a Packard sample oxidizer revealed the highest recovery and accuracy (93—100%), sample oxidizing by combustion without lyophilization the lowest recovery and accuracy (73—92%). Tissue solubilization by the commercially av…