Validation of energy expenditure and macronutrient oxidation measured by two new whole-room indirect calorimeters at the University of Kiel and biological determinants of bias

Objective Whole room indirect calorimeters (WRIC) measure the impact of energy flux or diet composition on energy and macronutrient balance. Two new WRICs located in Kiel (each with a volume of 24,282 L) are validated according to Room Indirect Calorimetry Operating and Reporting Standards (RICORS 1.0). Methods For technical validation, sixteen 10h-propane burns were analyzed to determine the precision of VO2 and VCO2 measurements and accuracy compared with predicted stoichiometric values. For biological validation, eight healthy participants (24.1±2.5 y; BMI 24.3±3.1 kg/m²) underwent four 24 h-protocols under h…