Fully automated immunoassay for quantitative determination of FXIII
Stephan, S.; Duwe, C.; Pechmann, L.; Althaus, H.; Ehm, M.; Fischer, B.; Hahn, M.; Schwarz, H.; Vitzthum, F.; Kappel, A. · Zeitschrift für Orthopädie und Unfallchirurgie · 2011 · Heft 02 · S. 105 bis 109
Bibliografische Angaben
Zusammenfassung
SummaryCoagulation factor XIII (FXIII) is essential for clot stabilization. Deficiency of FXIII is associated with a risk of bleeding and impaired wound healing. Substitution therapy with FXIII remedies for patients with low plasma levels of FXIII requires diagnostic quantification of the factor before and during therapy. Here, we describe a prototype of a preliminary research immunoassay for quantification of FXIII antigen on automated coagulation instruments. The prototype assay is based on a monoclonal antibody (mAb) directed against FXIII A chain, whereas the mAbs are coupled to latex particles. FXIII in a p…
