Beta 3-specific non-peptidic integrin inhibitor EMD409915 blocks PDGF-BB stimulated migration in HSC via inhibition of p38 signalling pathway

Aims: Platelet-derived growth factor (PDGF) is known to be the most potent stimulator of migration and proliferation for activated hepatic stellate cells (HSC) and the expression of PDGF-ß-receptors is increased during liver fibrosis. We aimed to investigate the effect of beta 3-integrin subunit blockade using a specific non-peptidic inhibitor EMD409915 on HSC migration, proliferation, and signal transduction. Materials and methods: Cell migration was assessed using a scratch assay. After wounding, cells were stimulated with PDGF-BB (10 ng/ml) with or without EMD409915 at 10–10M–10–6M. Rate of migration was meas…