Corticosteroid inhibits „organ defense“ mechanisms in the liver

The rat model of CCl4 induced acute damage offers the possibility to study some of the mechanism of „stress induction“, inflammation, damage and repair in the liver. Wistar rats were treated with intragastric administration of CCL4 dissolved in corn oil or with corn oil alone. Animals were sacrified at different time points after treatment and liver tissue was snap frozen in liquid nitrogen and stored at -80°C. RNA was extracted and analysed by Northern Blot or/and by realtime PCR. Early after CCl4 administration of the rats a increase of IFN-γ gene expression (maximum at 6 to 12 hours) was detectable. At the sa…