Collagenase P versus Liberase perfusion for large-scale isolation of primary liver cells from discarded human grafts

Aim: Human livers, discarded from transplantation due to excessive steatosis, fibrosis/cirrhosis or other reasons provide a suitable resource for primary human liver cells that can be used in artificial liver support systems. The therapeutic application of liver cells requires a high number and quality of cells. The outcome of the isolation procedure is strongly affected by the enzyme used for digestion of the organ. In this study we compared the effect of two different enzymes, crude collagenase and liberase, a highly purified collagenase, on the outcome of cell isolation process utilizing discarded grafts. Met…