Polymorphic genes of HSP-70–2, TNF-alpha, and CD14 in patients with acute pancreatitis

Aims: We hypothesized that the polymorphism of the TNF gene locus and of HSP70 may influence disease susceptibility and course in acute pancreatitis (AP). Moreover, the role of genetic polymorphism of the LPS receptor CD14 could not be excluded. Methods: DNA from 77 patients with AP was used for genetic experiments. The patients were grouped according to Ranson gene was determined by NcoI RFLP. The -308 polymorphism of TNF- polymorphism for HSP70–2 gene by PstI RFLP, and the CD14–159 polymorphism by melting point analysis. DNA was isolated from healthy controls (n=71). Results: No significant difference was note…