Specific T-cell responses to aspergillus fumigatus antigens

Invasive Aspergillosis is still a life-threatening infection in patients undergoing allogeneic stem cell transplantation (SCT). There is a growing body of evidence that T-cells play a major role in the immunological response to Aspergillus, and therefore, could be a potential therapeutic target in Aspergillosis. We performed a 3H-thymidine lymphoproliferation assay using different potential antigens of Aspergillus fumigatus. In order to generate Aspergillus-specific T-cells, the antigens with the highest proliferation indices (EC-SAB and 90 kDa catalase) were then used to stimulate 1.0×108 mononuclear cells from…