In depth analysis of aberrantly methylated genes detected by genome scanning in medulloblastoma and other malignancies of the CNS

Introduction: Silencing of genes by methylation is an important mechanism to inactivate tumor suppressor genes. Restriction Landmark Genomic Scanning using restriction enzyme combinations with NotI EcoRV or AscI EcoRV allow the scan of up to 4.000 CpG-Islands for aberrant methylation. Thus genes that might play a role in tumor development and metastasis can be identified. Results: The examination of 3 anaplastic ependymomas, 5 stPNET, 25 medulloblastomas and 7 cell lines by Restriction Landmark Genomic Scannning using NotI-EcoRV revealed 225 methylated CpG Islands. More than 30 methylated in at least 10 differen…