L-selectin expression defines a subpopulation of In vitro generated AML-reactive T-cells with superior anti-leukemic efficacy for adoptive immunotherapy

To determine the mechanisms by which adopive immunotherapy with leukemia–reactive cytotoxic T-cells (CTLs) could reduce AML lethality, a novel technique was developed to track, both leukemic blasts and adoptively transferred CTLs independently and simultaneously in vivo. Starting with splenocytes from eGFP (enhanced green fluorescent protein) transgeneic mice AML-reactive CTLs were genarated in vitro. Stable transfectants of the murine AML cell-line C1498 expressing the red fluorescent protein DSRed2 were obtained and injected into syngeneic mice. Three factors related to CTLs correlated with disease free surviv…