Increased COX2 expression enhances tumor-induced osteoclastic lesions in breast cancer bone metastasis

Objective: Metastastic dissimination depends on interaction between tumor and local host environment. In this study, we established a new mouse model to mimic the process of bone metastasis in breast cancer. Methods: TM40D-MB mouse mammary tumor cells were transplanted to the mammary glands of mice causing local tumore metastatic to bone. We compared the expression of high (TM40D-MB) versus low metastatic (TM40D) tumor cells using the Affymetrix microarray platform. Differentially expressed genes were confirmed by Northern-blot analysis. The COX2 gene was selected for functional studies. TM40D-COX2 and TM40D-C c…