CareLit Fachartikel
Anisoosmolarity alters HCV replication in the subgenomic hepatitis C virus replicon system
H Fey, B Sitek, T Gehrmann, K Stühler, HE Meyer, D Häussinger, A Erhardt · Zeitschrift für Gastroenterologie · 2009 · Heft 01
Dokument
809416
CareLit-ID
Jahr
2009
Publikation
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Metadaten
DOI
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Zusammenfassung
Background/Aim: The state of cellular hydration changes under the influence of hormones, cumulative substrate uptake and oxidative stress resulting in cellular shrinkage or swelling. We aimed at investigating whether anisoosmolarity has an effect on HCV replication. Methods: Huh9–13 cells carrying a subgenomic hepatitis C replicon, were cultured in either hypoosmotic (220 mosmol/l), isoosmotic (320 mosmol/l) or hyperosmotic media (420 mosmol/l) for 48h. Cytotoxicity was measured via XTT assay. Levels of genomic virus RNA were measured by RT-PCR and alterations in viral protein expression were measured by western…
Schlagworte
Anisotonicity
Cell shrinkage
Cell swelling
Hepatitis C
subgenomic Replicon
Zeitschrift für Gastroenterologie
