Expression of neuropeptide receptors in lung dendritic cells

Introduction: Pulmonary dendritic cells are in close contact to sensory nerve endings, which suggests neuroimmune interactions in the lung. Neuropeptides released by sensory nerves might affect dendritic cell activation, migration and antigen presentation. Therefore, in inflammatory conditions e.g. allergic asthma, the immune response upon allergen challenge might be in part due to a pathological changed neuroimmune interaction between sensory neurons and dendritic cells. Methods: CD11c+ cells were obtained by mouse lung digestion and MACS separation. Dendritic cells were sorted by FACS Aria as CD11c+ and MHCIIh…