Antibody-linked superparamagnetic iron oxide particles for detection of tumor cells at 3T
K Peldschus, H Ittrich, MG Kaul, A Horst, P Nollau, T Schlorf, U Schumacher, C Wagener, G Adam, C Nolte-Ernsting · RöFo - Fortschritte auf dem Gebiet der Röntgenstrahlen und der bildgebenden Verfahren · 2007 · Heft 03
Bibliografische Angaben
Zusammenfassung
Purpose: Cell adhesion molecules of the CEACAM family are over-expressed in epithelial tissue and microvessels of different carcinomas. The purpose was to detect these antigens with antibody-linked superparamagnetic iron oxide particles (SPIO) in an in-vitro model. Methods: SPIOs (Micromod, Warnemuende) were covalently linked to mouse antibodies, mAb T84.1 and IgG. Relaxivity (r2*) of SPIOs was determined at 3T using a 3D multi gradient-echo sequence. Specific immunoreactivity of functionalized particles was assessed by enzyme-linked immunosorbent assay (ELISA). CEACAM1 transfected CHO cells were incubated with…
