Role of Interleukin-6 in hepatic glucose production

Aims: Besides its function in inflammatory processes IL-6 is a potent player in metabolism. It is known to regulate insulin action and to stimulate lipolysis. The aim of our work is to elucidate the function of IL-6 in gluconeogenesis in hepatocytes. Methods: All experiments were conducted in Fao rat hepatoma cells (Fao). Cells were starved overnight in a serum and glucose free medium containing 2 mM lactate. On the following day cells were stimulated with various substances in a glucose- and serum-free medium containing 5 or 10 mM lactate. After indicated timepoints supernatant, protein and RNA was collected fo…