A dual role of the N-terminal FQQI motif in GLUT4 trafficking

Background and aims: Insulin stimulation of muscle and adipose cells results in a rapid and reversible translocation of GLUT4 from storage vesicles to the plasma membrane. A N-terminal phenylalanine-containing amino acid motif (F5QQI) is required for the endocytosis and subsequent sorting of GLUT4 into the vesicles. The F5A-mutant of GLUT4 accumulates at the cell-surface in an insulin-independent manner. In previous experiments, we identified the FQQI-motif as binding partner for the heterotetrameric adapter protein complexes AP-1 and AP-2. Our data suggested that the F5-motif is involved in endocytosis of the t…