Identification of loss of function RARRES1, PTGDR and FOXA1 as potential tumor markers by whole genome microarray analysis from laser capture microdissected colon epithelial cells

Background: In case of microarray data, the origin of signals (epithelium or stroma) cannot be distinguished. To avoid this obstacle, one should use homogeneous cell populations which can help us to get more exact information about the molecular background of tumors. Our aims were to identify mRNA expression patterns using LCM samples and to compare four different histological regions of colorectal tumor. Methods: From 6 Dukes B stage, moderately differentiated, left sided colorectal cancer, malignant and normal specimens were collected and frozen immediately after surgery. Using membrane mounted slides, 6µm thi…