The development of early human embryo under time-laps cinematography

Purpose: The aim of this study was to follow and record the early stages of development of a human embryo using time- lapse cinematography (TLC). Methods: Human ova were incubated in the chambers with stabilized temperature at 37° and pH at 7.45, and observed using an inverted microscope fitted with a CCD digital camera. Images were taken at 2-minute intervals for 5–6 days. A total of 30 consecutive images, corresponding to an hour of incubation, could then be replayed in 1 second. This allowed us to watch the development of embryos over 5–6 days in a few minutes. Results and discussion: We observed fertilizatio…