Expression and function of methylthioadenosine phosphorylase (MTAP) in chronic liver disease
B Czech, A Stevens, G Kirovski, M Saugspier, C Dorn, D Valletta, A Bosserhoff, P Oefner, C Hellerbrand · Zeitschrift für Gastroenterologie · 2011 · Heft 01
Bibliografische Angaben
Zusammenfassung
Methylthioadenosine phosphorylase (MTAP) the rate-limiting enzyme in the methionine and adenine salvage pathways catalyzes the phosphorylation of 5′-deoxy-5′-(methylthio)adenosine (MTA) which is a by-product of polyamine synthesis. The aim of this study was to assess MTAP expression and function during the progression of chronic liver disease. Methods and Results: MTAP mRNA and protein expression were reduced in the murine bile-duct ligation (BDL) model, and in addition, we detected reduced enzymatic MTAP activity. Accordingly, liquid chromatography tandem mass spectrometry revealed hepatic MTA accumulation and…
