TGF-beta mediates transcriptional repression of miR–29 a/b1 during liver fibrosis
J Huang, N Elfimova, A Noetel, W Amer, M Kwiecinski, HP Dienes, H Peter, M Odenthal · Zeitschrift für Gastroenterologie · 2012 · Heft 01
Bibliografische Angaben
Zusammenfassung
Background and aim: microRNA(miRNA) are small noncoding RNAs that play an important role in post-transcriptional inhibition of gene expression. Recent studies identified miR–29 as an antifibrotic miRNA, regulating collagen–1 and –4 expression in hepatic stellate cells (HSC). Additionally, the members of the miR–29 family were shown to be downregulated in livers from patients with advanced hepatic fibrosis. In the present approach, we studied the influence of the profibrotic mediator TGF-beta on promoter regulation of miR–29 expression. Methods: The expression of pri-miRNA29a/b1 and mature miR–29a/b1 was determin…
