The proximal TGR5 C-Terminus is required for membrane localization

TGR5 (Gpbar–1) is a plasma membrane-bound bile acid receptor, which is coupled to a stimulatory G-protein. The structure of TGR5 is unknown and it remains elusive which amino acids are important for receptor localization and function. Based on previous findings [1] that a C-terminal truncation variant (Q296) is retained in the endoplasmic reticulum and therefore nonfunctional, we tried to identify motifs in the TGR5 C-terminus important for receptor localization and function. Due to our previous findings we generated further truncation variants (D284, P290, A295, R297, Q300 and S310). The truncation variants D28…