Nuclear exclusion of TET1 is associated with loss of 5-hydroxymethylcytosine in IDH1 wildtype gliomas
T Müller, M Gessi, A Waha, LJ Isselstein, D Luxen, D Freihoff, J Freihoff, A Becker, M Simon, T Pietsch, A Waha · Klinische Pädiatrie · 2012 · Heft 04
Bibliografische Angaben
Zusammenfassung
IDH1 mutations are associated with a CpG island methylator phenotype in glioblastomas. Cells harboring IDH1 mutations produce 2-hydroxyglutarate inhibiting TET enzymes involved in the oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). Glioma tissues and cell lines were investigated by RT-PCR, immunohistochemistry and pyrosequencing for the expression of TET enzymes, 5hmC content and IDH1 status respectively. 61% of gliomas revealed no immunoreactivity for 5-hmC and no correlation was observed between IDH1 mutations and loss of 5hmC. The subcellular localization of TET1 showed remarkable diffe…
