Nanoparticles increase ciliary beat frequency in the murine trachea

Although the airway epithelium is the one of the first barriers that interacts with nanoparticles, the effects of nanoparticles on the airway epithelium are incompletely understood. We therefore examined the effects of two different nanoparticles on the epithelium of the mouse trachea. The trachea of Balb/c mice was explanted and incubate with nanoparticles up to 24h. Carbon black (Printex90, 10µg/ml, median size 160nm in solution) and silica particles (DQ12, 1µg/ml and 10µg/ml, median size of 600nm in solution) were used. Ciliary beat frequency and particle transport capacity were investigated by video microsco…