Role of protein turnover in NF-κB signalling: A systems biology study on primary hepatocytes

The cytokine tumor necrosis factor (TNF)-α is a key factor in the priming phase of liver regeneration since it activates the NF-κB signalling pathway, which primes hepatocyte for proliferation. In order to understand how TNFα-dependend NF-κB activation contributes to a cellular response in parenchymal liver cells, we aimed to generate a hepatocyte-specific mathematical model based on quantitative and time-resolved data of NF-κB pathway constituents after TNFα administration. For model parametrization, freshly isolated primary murine hepatocytes were treated with TNFα (10ng/ml) and protein levels (p65, IκBα), pho…