Transcriptional promoter regulation of the miR-29a/b1 gene in hepatoma cells

Background and aim: Recent studies have shown that members of the microRNA-29 (miR-29) family are dysregulated in several types of cancer. Dysregulation of miR-29 family members during hepatocarcinogenesis leads to a more malignant phenotype of hepatocellular carcinoma (HCC). Many potential targets of the miR-29 family were identified such as Mcl-1 and BCl2. In the present approach, we have studied promoter gene regulation of miR-29 expression. Methods: The expression of pri-miRNA29a/b1 was determined by Real-Time PCR in Huh7 after TGF-beta or phorbolester stimulation. The promoter of the miR-29a/b1 was analysed…