CareLit Fachartikel

High-throughput functional characterization of miRNAs in cytogenetically defined AML subgroups

R Jammal, S Emmrich, K Henke, J Kuipers, M Heuvel vd, D Reinhardt, JH Klusmann · Klinische Pädiatrie · 2013 · Heft 05

Dokument
822176
CareLit-ID
Jahr
2013
Publikation
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Metadaten
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Bibliografische Angaben

Zeitschrift
Klinische Pädiatrie
Autor:innen
R Jammal, S Emmrich, K Henke, J Kuipers, M Heuvel vd, D Reinhardt, JH Klusmann
Ausgabe
Heft 05 / 2013
Seiten
–
Erschienen: 2013-05-22 00:00:00
ISSN
0300-8630
DOI
10.1055/s-0033-1343630

Zusammenfassung

Acute Myeloid Leukemia (AML) is a common type of cancer in children and adolescents, characterized by recurrent cytogenetic abnormalities. MicroRNAs (miRNAs) have shown to play a major role in leukemogenesis. We conducted a functional anti-miR screening to complement a previous global gene expression profiling in cytogenetically characterized AML subgroups (MLL-rearranged, t(8;21), t(15;17)). Four leukemia cell lines with the corresponding karyotypes were transfected with 470 anti-miRs and cell viability was assessed after 72h and 96h. Bioinformatic analysis (B score calculation) revealed a total of 187 candidat…

Schlagworte

Klinische Pädiatrie