Bone marrow stromal cell conditioned media reduces endoplasmic reticulum stress in alveolar epithelial cells in vitro

Introduction: Idiopathic Pulmonary Fibrosis (IPF) is a chronic progressive lung disorder of unknown etiology. Evidence of endoplasmic reticulum (ER) stress has been found in lungs of IPF patients. In the present study we investigate possible effects of Bone Marrow Stromal Cell conditioned media (BMSC-cm) on ER stress response and wound healing in alveolar epithelial cells (AEC). Methods: Wounded A549 were treated with 3 µg of Tunicamycin for 24h to induce ER stress afterwards cells were treated with BMSC-cm or control media for 24h and 48h. qRT PCR was performed for analysis of ER stress markers: Grp78, XBP1 and…