Blockade of NLRP3 expression in primary hepatocytes by inhibitors of NF-κB signalling

The activation of NLRP3 inflammasome is performed in two steps. In a first step pro-inflammatory cytokines such as interleukin-1β (IL-1β) become activated by proteolytic cleavage of their proforms. As a consequence they stimulate the assembly of the NLRP3 inflammasome representing a large cytoplasmic multiprotein complex that process a large variety of danger and stress signals that are triggered by lipopolysaccharides (LPS), ATP or crystals that induce lysosomal destabilization [1]. In a previous study from our laboratory we showed that LPS stimulation of primary hepatocytes leads to a significant increase expr…