Bacterial DNA in non-leukocytic ascites, identification of risk factors
C Engelmann, S Krohn, D Prywerek, K Zeller, D Deichsel, F Janett, A Böhlig, S Böhm, T Berg · Zeitschrift für Gastroenterologie · 2014 · Heft 08
Bibliografische Angaben
Zusammenfassung
Background: As culture-dependent bacterial identification methods are still limited to capture colonisation or infection of ascites fluid we established a culture-independent PCR-based method for the detection, quantification and differentiation of bacterial DNA (bactDNA). This report aims to characterize risk factors for bactDNA identification in non-leukocytic ascites fluid (nlAF) and for progression to SBP. Methods: 299 nlAF samples of 142 patients were collected between 02/2011 and 12/2012. BactDNA was detected using real-time PCR with primers targeting the 16S-rRNA-gene and differentiated by direct sequenci…
