How is the potential of human neural stem cells in vitro and in vivo

Introduction: A number of gastrointestinal disorders such as gut motility or inflammatory diseases are caused by abnormalities of the enteric nervous system (ENS). Neural stem cells of the ENS have been proposed as an appropriate cell pool to provide an alternative therapeutic option for the unsatisfactory current treatments of these diseases. Methods: Neural stem cells from human postnatal gut biopsies were propagated as neurosphere-like bodies (NLBs). For in vitro implantation studies, NLBs were grafted into fetal slice cultures and aganglionic hindgut explants. For in vivo testing, immunodeficient CD-1 nude m…