Ulipristalacetate treatment of endometriosis and normal endometrial stromal cells inhibits cell proliferation

Question: Ulipristalacetate (UPA) has been previously demonstrated to inhibit the estradiol and progesterone induced proliferative response of normal endometrial cells in culture. We predicted that UPA would inhibit proliferation of endometriosis cells. Methodology: Stromal cells were isolated from a deep infiltrating endometriosis lesion and matched normal endometrium. Since the progesterone receptor (PGR) is the target of UPA, both primary cell lines were tested for gene expression of steroid hormone receptors using semi-quantitative real time PCR. For proliferation assays, cells were seeded at 50,000 cells pe…