Integration of exome sequencing and in vivo RNAi screening identifies new cancer genes in hepatocellular carcinoma

The recent improvements in the field of sequencing technologies facilitated profiling of whole (tumor) genomes, while in vivo RNAi screening allows for the detection and validation of new cancer genes in an unbiased fashion. Here we combined whole exomic next generation sequencing (NGS) of a single case of human hepatocellular carcinoma with in vivo RNAi screening to identify new protumorigenic driver genes of hepatocarcinogenesis. The selected case was characterized by a highly differentiated hepatocellular carcinoma (HCC) that manifested as a nodule in a premalignant Dysplastic Nodule (DN) in a cirrhotic patie…