Effect of inflammatory factors on air liquid interface epithelia
L Nonnenmacher, H Schmidt, VE Winkelmann, B Mizaikoff, M Huber-Lang, M Frick, P Dietl, OH Wittekindt · Pneumologie · 2016 · Heft 05
Bibliografische Angaben
Zusammenfassung
Inflammatory factors are known to disturb the transepithelial water transport in lung by altering the sodium transport. The epithelial sodium channel (ENaC) is regarded as a limiting factor for transepithelial water resorption. We investigated the effect of inflammatory factors on transepithelial transport across lung epithelia in vitro and addressed the question whether ENaC mediated mechanisms are involved. All experiments were performed on NCl-H441 cells cultivated at air-liquid interface (ALI) conditions as a well established model of the distal lung epithelium. IL13 (10 ng/ml), TNFα (10 ng/ml), LPS (200 ng/…
