Isolation and characterization of tumor infiltrating lymphocytes in primary human breast cancer

Introduction: Priming the immune system against tumor cells in breast cancer (BC) has recently become a major issue in developing personalized therapeutic strategies. Although the immunogenicity of BC is considered to be limited recent partial success of checkpoint-inhibitors emphasize the potential of immunotherapy in BC. Previous studies confirm the prognostic value of tumor infiltrating lymphocytes (TIL's) in BC, particularly in aggressive or metastatic forms, however the therapeutic significance of TIL's in BC is still poorly investigated. Methods: We established a method generating single cell suspensions o…