A telomerase dependent conditionally replicating adenovirus for treatment of hepatocellular carcinoma
T Wirth, L Zender, B Schulte, B Mundt, R Plentz, KL Rudolph, MP Manns, F Kühnel, S Kubicka · Zeitschrift für Gastroenterologie · 2015 · Heft 05
Bibliografische Angaben
Zusammenfassung
Background: The catalytic component of telomerase (hTERT) is not expressed in most primary somatic human cells whereas a large variety of cancer cells and especially hepatoma cell lines reactivate telomerase by transcriptional upregulation of hTERT. Methods/Results: In this study telomerase activity was determined in different cell lines via TRAP assays revealing high telomerase acitivity levels for all hepatoma cell lines but no activity in primary human hepatocytes. Consequently, a conditionally replicating adenovirus (hTERT-Ad) expressing E1A genes under control of a 255 bp-hTERT-promoter was constructed. An…
