Protein glutathionylation in respiratory epithelial cell lines

Glutathionylation is a reversible binding of glutathione (GSH) to SH-groups of proteins. Activity of many critical proteins (e.g. protein tyrosine phosphatase 1b, NF-kappaB, AP-1), involved in signal transduction during inflammation and oxidative stress, may be regulated through glutathionylation. Recently, a protocol using a biotinylated glutathione monoester to identify glutathionylated proteins was published (Sullivan DM et al, 2002). For our experiments, we modified this procedure and studied protein glutathionylation in respiratory epithelial cell lines (16HBE14o-, gift from Prof. D. Gruenert and A549, obta…