Role of brain CRF signaling pathways in development of postoperative gastric ileus in rats

Abdominal surgery (AS) delays gastric emptying (GE) through CRF receptors (CRF-R). CRF-R1 contributes to central CRF-induced sympathetic activation. CRF1 knockout mice did not develop gastric ileus. Aims: To characterize the CRF-R type involved in postoperative gastric ileus and the role of central sympathetic activation. Methods: Anestetized adult fasted male SD rats underwent midline celiotomy and 60s cecum palpation or anesthesia alone. CRF2 antagonsist, astressin2-B (Ass2B), was injected iv just before and the CRF1 antagonist, CP-154,526 (CP) was injected sc at 60min and icv at 30min prior to celiotomy. GE o…