Doxorubicin reduces telomerase activity in a human Ewing-Xenograft tumor
B Winter, C Lanvers-Kaminsky, S Koling, B Frodermann, Y Braun, KL Schäfer, RI Diallo, S Könemann, J Boos, N Willich, C Poremba, A Schuck · Klinische Pädiatrie · 2004 · Heft 05
Bibliografische Angaben
Zusammenfassung
Aim: For its almost selective expression in human tumours telomerase is a promising target for cancer treatment. We evaluated the effects of doxorubicin (DOX) on telomerase in the human Ewing sarcoma cell line STA-ET-1 in vitro and in nude mice in vivo. Methods: In vitro toxicity was determined by the MTT-assay. Tumour xenografts were analysed for proliferation, necrosis, and apoptosis by MIB-, HE-staining and TUNEL assay. Telomerase activity (TA) was examined by the TRAP assay and hTERT mRNA expression by real-time PCR. Results: In vitro along with growth inhibition DOX treatment reduced TA but the employed con…
